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Biological Activities of Various Solvent Extracts of Seomaeyakssuk (Artemisia argyi H.)
Dong-Gyu Kim, Jae Ran Kang, Jung-Hye Shin and Min-Jung Kang*
Namhae Garlic Research Institute, Namhae 52430, Korea
Received August 29, 2019 /Revised October 14, 2019 /Accepted October 29, 2019
Artemisia argyi H. has been used for centuries as a traditional medicine and food supplement in Asian countries. The objective of this study was to investigate the physiological activities of Artemisia argyi H. extracts prepared by butanol, chloroform, ethyl acetate, ethyl ether, hexane, and methanol extraction. We evaluated total phenol and flavonoid content, antioxidant activity, nitric oxide (NO) and reactive oxygen species (ROS) release, and osteoclastogenesis inhibition. The total phenolic and flavonoid contents were highest in the methanol extract (49.46 mg GAE/g and 24.32 mg QE/g, respectively). The methanol extracts also had the highest antioxidant activity (DPPH and ABTS radical scavenging ability and ferric reducing antioxidant power), while the hexane extract had the lowest. The release of NO and ROS was dose-dependently decreased by pre-treatment with all solvent extracts. At the same concentrations, the ethyl acetate and butanol extracts showed higher inhibition of NO and ROS production when compared with the other extracts. The butanol extract, at a concentration of 20 μg/ml, inhibited about 89% of the activity of the osteoclast marker, tartrate resistant acid phosphatase (TRAP). These results suggest that butanol extracts of Artemisia argyi H. may be effective natural medications for the prevention and treatment of osteoporosis.
Key words : Antioxidant effect, Artemisia argyi H., osteoclast, TRAP
Journal of Life Science 2019 Vol. 29. No. 11. 1241~1250 DOI : https://doi.org/10.5352/JLS.2019.29.11.1241